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Figure 4. MT2 activation by 100 μM 8MP suppressed calcium channel signaling via MAPK1 in DRG neurons (Part II). Real-time PCR and Western blotting from different groups with β-actin as an internal standard: Primary cultured DRG neurons were subjected to different treatments as follows: (A) Con siRNA group (treated with the control siRNA) and MAPK1 siRNA (treated with the MAPK1 siRNA), ***P<0.001 vs. Con siRNA group; (B) Con siRNA group (treated with the control siRNA), Con Cacna1b group (treated with the Cacna1b siRNA), <t>Cacna1e</t> siRNA group (treated with the Cacna1e siRNA), Grm1siRNA group (treated with the Grm1siRNA) and Grin2a siRNA group (treated with the Grin2a siRNA); n=8 in all groups.
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Figure 4. MT2 activation by 100 μM 8MP suppressed calcium channel signaling via MAPK1 in DRG neurons (Part II). Real-time PCR and Western blotting from different groups with β-actin as an internal standard: Primary cultured DRG neurons were subjected to different treatments as follows: (A) Con siRNA group (treated with the control siRNA) and MAPK1 siRNA (treated with the MAPK1 siRNA), ***P<0.001 vs. Con siRNA group; (B) Con siRNA group (treated with the control siRNA), Con Cacna1b group (treated with the Cacna1b siRNA), <t>Cacna1e</t> siRNA group (treated with the Cacna1e siRNA), Grm1siRNA group (treated with the Grm1siRNA) and Grin2a siRNA group (treated with the Grin2a siRNA); n=8 in all groups.
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Figure 4. MT2 activation by 100 μM 8MP suppressed calcium channel signaling via MAPK1 in DRG neurons (Part II). Real-time PCR and Western blotting from different groups with β-actin as an internal standard: Primary cultured DRG neurons were subjected to different treatments as follows: (A) Con siRNA group (treated with the control siRNA) and MAPK1 siRNA (treated with the MAPK1 siRNA), ***P<0.001 vs. Con siRNA group; (B) Con siRNA group (treated with the control siRNA), Con Cacna1b group (treated with the Cacna1b siRNA), Cacna1e siRNA group (treated with the Cacna1e siRNA), Grm1siRNA group (treated with the Grm1siRNA) and Grin2a siRNA group (treated with the Grin2a siRNA); n=8 in all groups.

Journal: Theranostics

Article Title: Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice.

doi: 10.7150/thno.19500

Figure Lengend Snippet: Figure 4. MT2 activation by 100 μM 8MP suppressed calcium channel signaling via MAPK1 in DRG neurons (Part II). Real-time PCR and Western blotting from different groups with β-actin as an internal standard: Primary cultured DRG neurons were subjected to different treatments as follows: (A) Con siRNA group (treated with the control siRNA) and MAPK1 siRNA (treated with the MAPK1 siRNA), ***P<0.001 vs. Con siRNA group; (B) Con siRNA group (treated with the control siRNA), Con Cacna1b group (treated with the Cacna1b siRNA), Cacna1e siRNA group (treated with the Cacna1e siRNA), Grm1siRNA group (treated with the Grm1siRNA) and Grin2a siRNA group (treated with the Grin2a siRNA); n=8 in all groups.

Article Snippet: The siRNA used for gene knocking down MAPK1 (sc-35336), Cacna1b (sc-42699), Cacna1e (sc-42703), Grm1 (sc-61027), Grin2a (sc-36084), RORα (sc-38863) or control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology.

Techniques: Activation Assay, Real-time Polymerase Chain Reaction, Western Blot, Cell Culture, Control